Quantitative determination of glucose in blood plasma by homonuclear proton decoupled water attenuation transverse relaxation Carr-Purcell-Meiboom-Gill (WATR-HDCPMG) NMR spectroscopy |
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Authors: | W Y Choy S C F Au-Yeung S Y Fan W L A K Chiu and C S Cockram |
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Institution: | (1) Department of Chemistry, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, HK;(2) Department of Medicine, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, HK |
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Abstract: | Glucose in 5% D2O/95% H2O solution was successfully determined quantitatively by measurements of the 1H NMR peak height (intensity) of the single peaks at δ(1H) = 5.22 and 4.64 ppm corresponding to the α-D and the β-D-glucose spectrum, respectively. The single peaks were obtained
from decoupling of the high field part of the AX spectrum of the α-D- or the β-D-glucose by incorporation of time shared homonuclear
decoupling in the WATR-CPMG method (WATR-HDCPMG) without re-attenuation of the water peak. The method was applied to the determination
of total glucose in blood plasma from human subjects undergoing oral glucose tolerance test (OGTT) in the teaching hospital.
The results compared favorably with those obtained from the standard glucose oxidase method obtained in a hospital pathology
laboratory. The accuracy of the results obtained using the WATR-HDCPMG method was within 3.5% of the glucose oxidation method.
Received: 5 January 1998 / Revised: 23 February 1998 / Accepted: 26 February 1998 |
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