Fluorescent Study of Human Blood Plasma Albumin Alterations Induced by Ionizing Radiation |
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Authors: | Elena M Kirilova Inta Kalnina Tija Zvagule Natalija Gabruseva Natalja Kurjane Irina I Solomenikova |
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Institution: | (1) Daugavpils University, Daugavpils, Latvia;(2) Riga Stradins University, Riga, Latvia;(3) Biomedical Research and Study Centre, University of Latvia, Riga, Latvia |
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Abstract: | The use of hydrophobic fluorescent probe ABM (benzanthrone derivative) and albumin autofluorescence allowed show conformational
alterations in Chernobyl clean-up workers blood plasma. Results obtained in 1996–1997 suggest that acidic expansion of plasma
albumin takes place. Latest data (2006–2008) result in splitting of albumin alterations onto two stages - acidic expansion
and N-F transition. The N-F transition is accompanied by the blue shift of fluorescence spectra and dehydration of tryptophanyl
region of albumin molecule. In 2007 obtained.patterns of ABM spectra had never been previously seen in examined healthy individuals
or patients with tuberculosis, multiple sclerosis, rheumatoid arthritis, etc. Patterns of ABM fluorescence spectra are associated
with conformational changes of blood plasma albumin. The use of probe ABM and albumin auto-fluorescence allowed show conformational
alterations in albumin of Chernobyl clean-up workers blood plasma. It is necessary to note that all investigated parameters
significantly differ in observed groups of patients. These findings reinforce our understanding that the blood plasma albumin
is a significant biological target of radiation. It may be concluded that fluorescence characteristics are representative
of radiation induced albumin alterations and its carrier function. |
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