Covalent imprinted polymer for selective and rapid enrichment of ractopamine by a noncovalent approach |
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Authors: | Tang Yi-Wei Fang Guo-Zhen Wang Shuo Li Jia-Le |
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Institution: | (1) Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science & Technology, Tianjin, 300457, China; |
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Abstract: | A novel molecularly imprinted polymer (MIP) for the separation and concentration of ractopamine (RAC) was prepared by a covalent
imprinting approach and the template was removed successfully by hydrolysis, so that four carboxylic acid groups were left
in the cavities and could specifically rebind RAC through noncovalent interaction: hydrogen bonding. The conditions for synthesis
of the MIP were optimized during the polymerization process, and a molar ratio of template–functional monomer complexes to
cross-linker of 1:3 was confirmed. The adsorption capacity of the MIP was 4.1-fold that of the nonimprinted polymer, and the
adsorption reaction reached equilibrium after 25 min at 50 mg L-1 concentration. The results of the competitive adsorption test showed that the MIPs had specific recognition ability for the
analyte RAC. In addition, the important factors affecting the efficiency of the method which was developed using the MIPs
as a solid-phase sorbent for separation and determination of RAC combined with high-performance liquid chromatography with
fluorescence detection were optimized. Under the optimum experimental conditions, the linear range of the calibration curve
in the method was 0.05-5 μg L-1 (R
2 = 0.98) and the limit of detection (signal-to-noise ratio of 3) was 0.01 μg L-1. The proposed method was applied to determination of RAC in spiked feedstuffs and urine samples, with recoveries ranging
from 74.17 to 114.46% and relative standard deviation (n = 3) below 4.55 in all cases. |
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