A novel method to fabricate patterned bilayer lipid membranes |
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Authors: | Han Xiaojun Critchley Kevin Zhang Lixin Pradeep Singh N D Bushby Richard J Evans Stephen D |
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Affiliation: | School of Physics and Astronomy, and Self-Organising Molecular Systems (SOMS) Centre, University of Leeds, LS2 9JT Leeds, United Kingdom. |
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Abstract: | We introduce a new method for forming tethered bilayer lipid membranes on surfaces patterned using a photocleavable self-assembled monolayer (SAM). A SAM terminated with a hydrophobic fluorocarbon residue was bound to a gold surface through a link containing a photocleavable ortho-nitrobenzyl moiety. Hydrophilic regions were produced by irradiation with soft UV (365 nm) through a photomask. The patterned surface was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. Tethered bilayer lipid membranes with well-defined bilayer and monolayer regions were then formed by exposure to egg PC vesicles. The membranes had resistance and capacitance values of 0.52 MOmega.cm2 and 0.83 microF.cm-2, respectively. |
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