Towards molecular-imprint based SPE of local anaesthetics |
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| Authors: | L. I. Andersson M. Abdel-Rehim L. Nicklasson L. Schweitz S. Nilsson |
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| Affiliation: | (1) Faculty of Pharmacy, Semmelweis University, Department of Pharmacognosy, üllői út 26, 1085 Budapest, Hungary;(2) Institute of Chemistry, Hungarian Academy of Sciences, Chemical Research Center, Pusztaszeri út 59-67, 1025 Budapest, Hungary;(3) Pharmaceutical Biology, Institute of Pharmacy, Friedrich-Schiller University, Semmelweis Str. 10, 07743 Jena, Germany |
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| Abstract: | Summary Solidago canadensis L., Canadian goldenrod (Asteraceae) has been used in European phytotheraphy for centuries as a component of urological and antiphlogistical remedies. High-performance liquid chromatography (HPLC) coupled with diode-array detection (DAD) and online mass spectrometry (MS) has been used for the separation and quantification of phenolics (chlorogenic acid, caffeic acid, kaempferol-3-O-α-L-rutinoside (nicotiflorin), quercetin-3-O-β-D-rutinoside (rutin), quercetin-3-O-β-D-galactoside (hyperoside), quercetin-3-O-β-D-glucoside (isoquercitrin), quercetin-3-O-β-D-rhamnoside (quercitrin), kaempferol-3-O-α-L-rhamnoside (afzelin) and quercetin from Solidaginis herba. Extracts have been obtained using different technologies. Three aqueous and three alcoholic extracts were studied separately. Reversedphase high-performance liquid chromatography separation of polyphenols on octadecyl sorbent Hypersil was performed, using acetonitrile: acetic acid 2.5 v/v % as eluent in gradient elution. Our results confirm previous reports concerning the presence of several flavonoids. Quantification of the main quercetin glycosides in pharmaceuticals is also reported. Presented at Balaton Symposium '01 on High-Performance Separation Methods, Siófok, Hungary, September 2–4, 2001 |
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| Keywords: | Column liquid chromatography Mass spectrometry Solidago canadensis L. Flavonol glycosides Identification and quantification |
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