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Rapid Purification and Identification of Osteopontin from Human Milk by HPLC Coupled to FT-ICR-MS |
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| Authors: | SHI Lei YANG Tie-zheng |
| Institution: | [1]College of Public Health, Jilin University, Changchun 130021, P. R. China [2]Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, P. R. China [3]Laboratory for Biological and Medical Mass Spectrometry, BMC, Uppsala University, Box 583, 75123 Uppsala, Sweden |
| Abstract: | Milk as a key element for infant nutrition represents the only source of feeding for newborns and infants, breast-feeding milk normally contains several bioactive proteins or peptides useful for the development of the immune system that protects infants from diseases. Osteopontin(OPN) plays a distinct role during the processes of lactation. Some studies on OPN isolated and purified from the human milk via HPLC on SCX and C4 columns adopted biological mass spectrometry. After digesting the purified OPN sampl... |
| Keywords: | Osteopontin(OPN) FT-ICR-MS Purification Identification |
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