Separation of oligonucleotides on novel monolithic columns with ion-exchange functional surfaces. |
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Authors: | D Sykora F Svec J M Fréchet |
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Institution: | Department of Chemistry, University of California, Berkeley 94720-1460, USA. |
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Abstract: | Porous monolithic columns have been prepared by the direct free radical copolymerization of glycidyl methacrylate and ethylene dimethacrylate within the confines of a 50x8 mm I.D. chromatographic column in the presence of porogens. The epoxide groups of these monoliths were modified to different extents by reaction with diethylamine to afford 1-N,N-diethylamino-2-hydroxypropyl functionalities useful for ion-exchange chromatography. Following characterization of the monoliths, the columns were tested in the chromatographic separation of a homologous series of oligodeoxyadenylic pd(A)(12-18)] and oligothymidylic acids d(pT)(12-24)] at different flow-rates. Very good separations of the oligonucleotides were achieved even at the high flow-rate of 4 ml/min. |
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