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| 基于树状高分子固定DNA的电化学生物传感器的研究 | |
| 引用本文： | 时伟杰,艾仕云,李金焕,朱鲁生.基于树状高分子固定DNA的电化学生物传感器的研究[J].分析化学,2008,36(3):335-338. |
| 作者姓名： | 时伟杰 艾仕云 李金焕 朱鲁生 |
| 作者单位： | 1. 山东农业大学化学与材料科学学院,泰安,271018;山东农业大学,资源与环境学院,泰安,271018 2. 山东农业大学化学与材料科学学院,泰安,271018 3. 山东农业大学,资源与环境学院,泰安,271018 |
| 基金项目： | 国家自然科学基金(No.Y2047022,20775044)，山东省自然科学基金(No.Y2006B20)资助项目 |
| 摘 要： | 用电化学氧化法使玻碳电极表面氧化生成羧基,利用偶联活化试剂将1.0G树状高分子(PAMAM)固定在玻碳电极表面,并通过共价结合固定ssDNA。以亚甲基蓝为指示剂,采用循环伏安法、示差脉冲伏安法等电化学方法对DNA电化学生物传感器进行了表征。结果发现,通过亚甲基蓝与双链dsDNA作用的氧化还原电流的变化,可以识别和定量检测溶液中互补的ssDNA片段。经过条件优化,本法测定DNA的浓度线性范围为2×10-9～2×10-7mol/L,检出限为1×10-9mol/L。 |
| 关 键 词： | 聚酰胺-胺树状高分子 脱氧核糖核酸 杂交 亚甲基蓝 |
| 收稿时间： | 9 June 2007 |
| 修稿时间： | 2007年7月9日 |
Electrochemical Biosensor Based on Dendrimer Immobilized Deoxyribonucleic Acid |
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| Shi Wei-Jie,Ai Shi-Yun,Li Jin-Huan,Zhu Lu-Sheng.Electrochemical Biosensor Based on Dendrimer Immobilized Deoxyribonucleic Acid[J].Chinese Journal of Analytical Chemistry,2008,36(3):335-338. | |
| Authors: | Shi Wei-Jie Ai Shi-Yun Li Jin-Huan Zhu Lu-Sheng |
| Institution: | aCollege of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, China;bCollege of Resources and Environment, Shandong Agricultural University, Taian 271018, China |
| Abstract: | A novel DNA electrochemical biosensor was prepared by immobilizing the first generation polyamidoamine (G1 PAMAM) dendrimer on the glassy carbon electrode (GCE) surface by coupled activation agent, which was treated via electrochemical oxidation. Using methylene blue as the indicator, the DNA electrochemical biosensor was characterized by cyclic voltammetry and differential pulse voltammetry. The results indicated that the complementary ssDNA segment in the solution could be recognized and detected through mensurating the oxidation- deoxidation peaks current of embed methylene blue in dsDNA. The linear range for the determination of target DNA is from 2 × 10−9 to 2 × 10−7 M, and the detection limit is 1 × 10−9 M. |
| Keywords: | Polyamidoamine dendrimers deoxyribonucleic acid hybridization methylene blue |
| 本文献已被 CNKI 万方数据 ScienceDirect 等数据库收录！ | |