Colorimetric test-systems for creatinine detection based on composite molecularly imprinted polymer membranes |
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Authors: | TA Sergeyeva LA Gorbach EV Piletska SA Piletsky OO Brovko LA Honcharova OD Lutsyk LM Sergeeva OA Zinchenko AV El'skaya |
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Institution: | 1. Institute of Molecular Biology and Genetics, 150 Zabolotnogo Str., 03680 Kiev, Ukraine;2. Institute of Macromolecular Chemistry, 48 Kharkivske Shosse, 02160 Kiev, Ukraine;3. Cranfield Health, Cranfield University, Cranfield, Bedfordshire MK43 0AL, UK |
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Abstract: | An easy-to-use colorimetric test-system for the efficient detection of creatinine in aqueous samples was developed. The test-system is based on composite molecularly imprinted polymer (MIP) membranes with artificial receptor sites capable of creatinine recognition. A thin MIP layer was created on the surface of microfiltration polyvinylidene fluoride (PVDF) membranes using method of photo-initiated grafting polymerization. The MIP layer was obtained by co-polymerization of a functional monomer (e.g. 2-acrylamido-2-methyl-1-propanesulfonic acid, itaconic acid or methacrylic acid) with N, N′-methylenebisacrylamide as a cross-linker. The choice of the functional monomer was based on the results of computational modeling. The creatinine-selective composite MIP membranes were used for measuring creatinine in aqueous samples. Creatinine molecules were selectively adsorbed by the MIP membranes and quantified using color reaction with picrates. The intensity of MIP membranes staining was proportional to creatinine concentration in an analyzed sample. The colorimetric test-system based on the composite MIP membranes was characterized with 0.25 mM detection limit and 0.25–2.5 mM linear dynamic range. Storage stability of the MIP membranes was estimated as at least 1 year at room temperature. As compared to the traditional methods of creatinine detection the developed test-system is characterized by simplicity of operation, small size and low cost. |
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Keywords: | Molecularly imprinted polymers Molecularly imprinted polymer membranes Grafting polymerization Test-system Creatinine |
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