Paper-based solid-state electrochemiluminescence sensor using poly(sodium 4-styrenesulfonate) functionalized graphene/nafion composite film |
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Authors: | Yuanhong Xu Baohua LouZhaozi Lv Zhixue ZhouLibing Zhang Erkang Wang |
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Affiliation: | State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China |
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Abstract: | Herein, highly efficient solid-state ECL sensor was introduced for the first time onto the screen printed electrodes of the paper-based chips (PCs) based on the composite film of poly(sodium 4-styrenesulfonate) functionalized graphene (PSSG) and Nafion. Attributed to the cooperative characteristics of both PSS and graphene, PSSG ensured both effective Ru(bpy)32+ immobilization and fast electron transfer of Ru(bpy)32+ in the composite film. The ECL behaviors at the developed sensor were investigated using tripropylamine as a representative analyte and low detection limit (S N−1 = 3) of 5.0 nM was obtained. It also exhibited more excellent reproducibility (relative standard deviations of 0.63% for continuous 45 cycles) and long-term stability (∼80% of its initial ECL intensity could be retained over 3 months). More importantly, assisted by the developed ECL sensor, discrimination of 1.0 nM single-nucleotide mismatch in human urine matrix could be realized on the PCs for the first attempt. Thus, the developed sensor was confirmed with the advantages of highly sensitivity, long-term stability, simplicity, low cost, disposability, high efficiency and potential applicability. |
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Keywords: | AFM, Atomic force microscopy CC, completely complementary sequence CE, counter electrode CV, cyclic voltammetry EC, electrochemical ECL, electrochemiluminescence EIS, electrochemical impendence spectroscopy ΔEp, potential difference between the anodic and cathodic peaks GO, graphene oxide ipa, oxidation peak current MA, complementary sequence with single-nucleotide (adenine, A) mismatch MC, complementary sequence single-nucleotide C mismatch MT, complementary sequence single-nucleotide T mismatch M2, complementary sequence with two-nucleotides (GA) mismatch M3, complementary sequence with three-nucleotides (GAG) mismatch M4, complementary sequence with four-nucleotides (AGAG) mismatch P, p53 gene sequence segment PBS, phosphate buffer solution PCs, paper-based chips PMT, photomultiplier tube PSS, poly(sodium 4-styrenesulfonate) PSSG, poly(sodium 4-styrenesulfonate) functionalized graphene RE, reference electrode RSDs, s relative standard deviations Ru(bpy)32+, Tris(2,2&prime -bipyridyl)ruthenium(II) SPE, screen-printed electrode TPA, tripropylamine v1/2, square root of the scan rate WE, working electrode |
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