Effect of quality characteristics of single sample preparation steps in the precision and coverage of proteomic studies—A review |
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Authors: | Thomas Krüger Thomas Lehmann Heidrun Rhode |
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Institution: | 1. Institute of Biochemistry I, Nonnenplan 2, Universitätsklinikum Jena, 07740 Jena, Germany;2. Institute of Medical Statistics, Computer Sciences and Documentation, Bachstraße 18, Universitätsklinikum Jena, 07740 Jena, Germany |
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Abstract: | Proteomic profiling and biomarker search are analytical tools as many other. Nevertheless, in the proteomic discovery phase considerable sample fractionation is inevitable before readout. Since these procedures are of notable complexity, proteomic tools need in particular analytical quality validation standards as prevail for other analytical methods. With acceptance of the rule of error propagation the values of imprecision and yield of each preparation step determine overall reproducibility and therewith information harvest of a propagated method series. Thereto, we examined recent proteomic reports with reproducibility data and with parallelization, and automation approaches. Based on the data available from literature it is highly probable, that at least a part of current proteomic platforms actually suffer from high technical variance. |
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Keywords: | AEC anion exchange chromatography ConA concanavalin A CV coefficient of variation = relative standard deviation EIC extracted ion chromatogram FFE free flow electrophoresis HP high-performance LAC lectin affinity chromatography MARS multiple affinity removal system MCE multichannel electrolyte PF2D protein fractionation by 2D liquid chromatography RP reversed phase SOP standard operation procedure SXC strong cation exchange chromatography WGA wheat germ agglutinin |
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