Peptide-induced patterning of gold nanoparticle thin films |
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Authors: | Hassan M. BortehNicholas J. Ferrell Randall T. ButlerSusan V. Olesik Derek J. Hansford |
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Affiliation: | a Biophysics Graduate Program, The Ohio State University, Columbus, OH 43210, United States b Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, United States c Department of Material Science and Engineering, The Ohio State University, Columbus, OH 43210, United States d Department of Chemistry, The Ohio State University, Columbus, OH 43210, United States |
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Abstract: | In this work, the use of patterned proteins and peptides for the deposition of gold nanoparticles on several substrates with different surface chemistries is presented. The patterned biomolecule on the surface acts as a catalyst to precipitate gold nanoparticles from a precursor solution of HAuCl4 onto the substrate. The peptide patterning on the surfaces was accomplished by physical adsorption or covalent attachment. It was shown that by using covalent attachment with a linker molecule, the influence of the surface properties from the different substrates on the biomolecule adsorption and subsequent nanoparticle deposition could be avoided. By adjusting the reaction conditions such as pH or HAuCl4 concentration, the sizes and morphologies of deposited gold nanoparticle agglomerates could be controlled. Two biomolecules were used for this experiment, 3XFLAG peptide and bovine serum albumin (BSA). A micro-transfer molding technique was used to pattern the peptides on the substrates, in which a pre-patterned poly(dimethylsiloxane) (PDMS) mold was used to deposit a lift-off pattern of polypropylmethacrylate (PPMA) on the various substrates. The proteins were either physically adsorbed or covalently attached to the substrates, and an aqueous HAuCl4 solution was applied on the substrates with the protein micropatterns, causing the precipitation of gold nanoparticles onto the patterns. SEM, AFM, and Electron Beam Induced Current (EBIC) were used for characterization. |
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Keywords: | Gold nanoparticles Patterned deposition Peptide-induced deposition Protein attachment Soft lithography |
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