Post TLC developing technique for tyrosinase inhibitor detection |
| |
Authors: | Wangthong Sakulna Tonsiripakdee Ilhum Monhaphol Thitinun Nonthabenjawan Ratchanee Wanichwecharungruang Supason Pattanaargson |
| |
Institution: | Department of Chemistry, Faculty of Science, Chulalongkorn University, Payatai, Bangkok 10330, Thailand. |
| |
Abstract: | Post TLC developing technique was developed to detect substances which can inhibit tyrosinase activity. The method involved spraying the TLC plate or chromatographic paper containing sample spot(s) with tyrosinase and l-tyrosine solutions successively. A positive result could be visualized directly as white spot(s) against a brownish-purple background. The method can either be used as a quick screening method for tyrosinase inhibitor detection or a guiding procedure for an isolation of tyrosinase inhibitors from mixtures or natural product extracts. The technique is sensitive enough to give a clear result in the presence of only 6 ng glabridin. |
| |
Keywords: | tyrosinase inhibitor thin layer chromatography natural product extract |
本文献已被 PubMed 等数据库收录! |