| 化学发光酶联免疫分析测定血清中抗DNA抗体 |
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| 引用本文： | 章竹君,邹克渭.化学发光酶联免疫分析测定血清中抗DNA抗体[J].高等学校化学学报,1994,15(6):832-835. |
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| 作者姓名： | 章竹君 邹克渭 |
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| 作者单位： | 陕西师范大学分析科学研究所 |
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| 摘 要： | 建立起适于临床应用的抗ＤＮＡ抗体化学发光酶联免疫分析法。该法精密度良好，相对标准偏差为２．４％。比ＥＬＩＳＡ法更加简便、经济、省时，同时提高了灵敏度（８倍）和血清的阳性检出率。探讨了检测系统中对碘苯酚增强鲁米诺－过氧化氢－辣根过氧化物酶化学发光反应机理。
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| 关 键 词： | 化学发光 ELISA 抗DNA抗体 血清 |
CL-ELISA Determination of DNA Antibody in Serum |
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| ZHANG Zhu-Jun,ZOU Ke-Wei,FENG Man-Liang.CL-ELISA Determination of DNA Antibody in Serum[J].Chemical Research In Chinese Universities,1994,15(6):832-835. |
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| Authors: | ZHANG Zhu-Jun ZOU Ke-Wei FENG Man-Liang |
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| Abstract: | A method for determination of the DNA antibody in serum was established based on chemiluminescence enzyme-linked immunosorbant assay(CL-ELISA).The DNA antigen is immobilized on the inner surface of polystyrene cup,then reacts with DNA antibody in serum sample,only specific antibody binds to antigen to form Ag-Ab HRP labeled anti-HuIgG reacts with DNA antibody molecules in the complex to form second Ag-Ab,finally determine the label with p-IP enhanced luminol-H2O2-HRP chemiluminescence system.The clinical applications show that present method has a good correlation with ELISA.The correlation coefficient is 0.958.It is more sensitive,convenient and economical than ELISA. |
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| Keywords: | Chemiluminescence ELISA DNA antibody Systemic lupus erythematosus |
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