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Ceramic Carbon/Polypyrrole Materials for the Construction of Bienzymatic Amperometric Biosensor for Glucose
Authors:Fa Ming TIAN  Lian De ZHU  Bo XU  Guo Yi ZHU Laboratory of Electroanalytical Chemistry  Changchun Institute of Applied Chemistry  Chinese Academy of Sciences  Changchun
Affiliation:Fa Ming TIAN,Lian De ZHU,Bo XU,Guo Yi ZHU* Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022
Abstract:A novel amperometric glucose biosensor was constructed by electrochemical formation of a polypyrrole (PPy) membrane in the presence of glucose oxidase (GOD) on the surface of a horseradish peroxidase (HRP) modified ferrocenecarboxylic acid (FCA) mediated sol-gel derived ceramic carbon electrode. The amperometric detection of glucose was carried out at +0.16 V (vs. SCE) in 0.1 mol/L phosphate buffer solution (pH 6.9) with a linear response range between 8.010-5 and 1.310-3 mol/L of glucose. The biosensor showed a good suppression of inter- ference and a negligible deviation in the amperometric detection.
Keywords:Biosensor for glucose   polypyrrole   ceramic carbon electrode   sol-gel.
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