On-line simultaneous removal of human serum albumin and enrichment of doxazosin using a weak cation-exchange monolithic column |
| |
Authors: | Yang Gengliang Liu Haiyan Zhang Yihua Wang Sumin Yin Junfa Yin Boying Chen Yi |
| |
Institution: | Institute of Chemistry, Chinese Academy of Science, Beijing, China. ygl@mail.hbu.edu.cn |
| |
Abstract: | A weak cation-exchange monolithic column was prepared by modifying the GMA-EDMA (glycidyl methacrylate-co-ethylene glycol dimethacrylate) monoliths with ethylenediamine and monochloracetic acid. The properties of the column were investigated; the column exhibited the ability of low backpressure and fast analysis. Using this monolithic column, trace doxazosin in human serum albumin (HSA) solution and plasma samples has been on-line tested, the extraction efficiency and the maximum loading capacity of the monolithic column were obtained. The results showed that the monolithic column could realize deproteinization and trace drug enrichment simultaneously in the HSA solution and human plasma, which provided a simple, cheap, effective and friendly to environment method for assaying drugs in the blood. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|