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Electrical and optical characterization of thrombin-induced permeability of cultured endothelial cell monolayers on semiconductor electrode arrays |
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| Authors: | H Hillebrandt A Abdelghani C Abdelghani-Jacquin M Aepfelbacher E Sackmann |
| Institution: | Physik Department (E22), Institut für Biophysik, Technische Universit?t München, James Franck Strasse, 85748 Garching, Germany, DE Uniti de Recherche de Physique des Semiconducteurs, IPEST, BP51, 2070 La Marsa, Tunisia, TN Max von Pettenkofer Institut für Medizinische Mikrobiologie, Universit?t München, Pettenkoferstr. 9a, 80336 München, Germany, DE |
| Abstract: | Impedance spectroscopy and phase-contrast microscopy are combined to monitor the electrical and morphological properties of human umbilical vein endothelial cell monolayers. The cells were cultured on optically transparent indium-tin-oxide (ITO) semiconductor electrode arrays coated with collagen IV, and the effect of the inflammatory mediator thrombin on monolayer permeability was monitored in real time. ITO electrodes provide several advantages for these kinds of experiments, because they are optically transparent, polarizable and highly sensitive due to the absence of insulating oxide layers. A qualitative correlation between the thrombin-induced gap formation and the electrical parameters of the cell layer is established. Received: 8 August 2000 / Accepted: 2 March 2001 / Published online: 20 June 2001 |
| Keywords: | PACS: 73 40 Mr 78 66 Qn 87 18 Ed |
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