Absorption spectra of photosensitized human fat tissue |
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Authors: | I Yu Yanina G V Simonenko V I Kochubey and V V Tuchin |
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Institution: | 1.Saratov State Institute,Saratov,Russia;2.University of Problems of Precision Mechanics and Control,Russian Academy of Sciences,Saratov,Russia |
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Abstract: | We study experimentally the optical characteristics of adipose tissue in vitro in its dye sensitization—by indocyanin green
and brilliant green. We conducted experiments on a PerkinElmer Lambda 950 spectrophotometer in the spectral range of 250–900
nm. From an analysis of the measured absorption spectra, we determine the shifts in the maximum of the absorption band for
the studied photosensitizers in adipose tissue in comparison to their spectra in solutions. These shifts are explained by
the bonding of photosensitizer molecules with the protein matrix of adipose tissue. We find the relative monomer and dimer
concentrations of dye molecules in solutions and stained samples. |
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Keywords: | |
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