基于金胶的选择性聚集实现p53基因的电化学超灵敏检测

介绍了一种利用金胶的选择性聚集实现信号扩增的超灵敏的电化学方法, 用于人类p53肿瘤抑制剂基因的检测. 在实验中, 根据p53基因的序列设计了能特异性检测p53肿瘤抑制剂基因的二段探针, 在一段探针上固定磁性颗粒以捕获并富集目标基因, 同时在另一段探针上标记金纳米颗粒作为检测信标. 另外, 通过硫代三聚氰酸和金纳米颗粒的自组装作用, 形成金纳米颗粒和硫代三聚氰酸的网状结构, 获得金纳米颗粒的选择性聚集, 实现信号扩增. 用此法检测目标p53野生型DNA, 最低检测限为2.24×10-17 mol/L, 同时进一步研究了该探针对p53野生型和一碱基错配的突变型的选择性.

Ultrasensitive Electrochemical Assay for the Detection of p53 Sequence Based on the Aggregation of Gold Nanoparticles

An ultrasensitive and high specific electrochemical assay for the detection of human tumor suppression p53 gene has been developed.Two DNA sequences were designed,and the combined sequences were complementary to that of the target p53 sequence,one was immobilized on the magnetic nanoparticles for capturing the target p53 sequence,the other was labeled with gold nanoparticles as probe.The ultrasensitive detection was achieved by the aggregation of gold nanoparticles with introducing thiocyanuric acid into th...