浊点萃取-分光光度法测定水样中痕量结晶紫

提出了浊点萃取-分光光度法测定水样中结晶紫的新方法,研究了非离子表面活性剂Triton X-114浊点萃取的最佳条件,如pH、试剂用量、平衡时间和温度等。结晶紫的最大吸收波长为579 nm,标准曲线的线性范围是32～700 ng/mL,检出限是9.8 ng/mL,富集倍率为20。结晶紫的浓度在0.2和0.5μg/mL时的相对标准偏差分别为2.5%和1.7%(n=8)。应用本方法测定水样中的痕量结晶紫,平均回收率95.2%～98.1%。

Determination of trace amount of crystal violet by spectrophotometry after cloudpoint extraction

A new method for the determination of trace amount of crystal violet from aqueous solutions by spectrophotometry after cloud point extraction has been proposed. The optimum extraction and reaction conditions, such as pH, reagents concentration and effect of time and temperature, have been studied with the nonionic surfactant triton X-114. The maximum absorption wavelength of crystal violet is 579 nm. Linearity was obeyed in the range of 32～700 ng/mL with the limit of detection of 9.8 ng/mL. The enrichment factor was 20 for crystal violet. The relative standard deviations for the simultaneous determination of 8 test samples at the levels of 0.2 and 0.5 μg/mL were 2.5% and 1.7%, respectively. The proposed method was successfully applied to the determination of trace amount of crystal violet in real water samples with the recoveries of 95.2%～98.1%.