Determination of protein phosphorylation sites by mass spectrometry: a novel electrospray-based method |
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| Authors: | Alverdi Vera Di Pancrazio Francesca Lippe Giovanna Pucillo Carlo Casetta Bruno Esposito Gennaro |
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| Institution: | Department of Biomedical Sciences and Technologies, Center of Excellence M.A.T.I., University of Udine, P. le Kolbe 4, 33100 Udine, Italy. |
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| Abstract: | Several methods are used to identify protein phosphorylation sites. We report a novel electrospray-based method for the determination of phosphorylation sites by mass spectrometry, using two different declustering potential values. This method allows one to obtain, with a single liquid chromatography/mass spectrometry (LC/MS) run, the pattern with either the phosphorylated or the unphosphorylated species of a protein tryptic digest, that can be further analyzed by tracing back the origin of each HPO3-deprived form using the capabilities of tandem mass spectrometers. |
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