Fast purification process optimization using mixed-mode chromatography sorbents in pre-packed mini-columns |
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Authors: | Brenac Brochier Virginie Schapman Anthony Santambien Patrick Britsch Lothar |
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Institution: | Pall BioSepra, PALL France, 48 Avenue des Genottes, Cergy St. Christophe, France. virginie_brochier@europe.pall.com |
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Abstract: | Pre-packed MediaScout MiniChrom columns of 2.5, 5 and 10 mL were investigated for screening three mixed-mode chromatography sorbents (HEA, PPA and MEP HyperCel). Packing performance was of good quality and the three sorbents displayed higher capacity than traditional HIC sorbents in physiological-like conditions. Each sorbent offered a unique selectivity. Bovine beta-lactoglobulin was partially purified after loading milk whey directly on HEA HyperCel sorbent. The combination of small pre-packed columns and SELDI-MS appeared to be a valuable strategy for high-throughput screening of chromatography sorbents and for enabling rapid process development and optimization. |
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