Investigation of water environments in a C18 bonded silica phase using 1H magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy |
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Authors: | Coen M Lindon J C Gavaghan C Holmes E Humpfer E Wilson I D Stanley P D Nicholson J K |
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Institution: | Biological Chemistry, Biomedical Sciences Division, Imperial College of Science, Technology and Medicine, Sir Alexander Fleming Building, South Kensington, London, UK SW7 2AZ. |
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Abstract: | High resolution 1H magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectra have been obtained on typical C18 bonded silicas used in chromatographic solid-phase extraction separations. It has been shown for the first time that water molecules distributed in distinct physico-chemical environments within the chromatographic system can be detected directly using a simple 1H MAS NMR measurement. The resonances assigned to water protons in differing physico-chemical environments have distinct chemical shifts, line widths, relaxation times (T1 and T2) and also exhibit temperature dependent coalescence behaviour. This novel MAS approach may lead to a better understanding of the environments of other analytes in mixtures during such separations. |
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