柱上衍生反相色谱法测定金属配合物的稳定常数

提出一种计算金属配合物稳定常数的新方法。应用住上衍生反相高效液相色谱法，以二乙基二硫代氨基甲酸钠为配体，测定金属配合物的保留时间，用以推导金属配合物的稳定常数。

Determination of Stability Constants of Metal Complexes by On-Column Derivatization Reversed Phase High Performance Liquid Chromatography

The stability constant of metal complexes is an important chemical parameter. There are many methods for its determination , but few analyst has calculated it with high performance liquid chromatography (HPLC). In the present paper a new model for determining the stability constant of metal complexes with on-column derivatization reversed phase HPLC has been proposed where complexation forming reactions are taking place in the mobi1e phase. Based on the retention mechanism of neutral complexes, the equation for calculation is deduced. It has been applied to some cations (Hg2 ,Pb2 , Ni2 ,Cu2 ,Ag , Hg )-DDTC complexes. The results are in agreement with those given in the literature. Then, a simple, practical and rapid graphic method is studied for monovalent metal complexes. The stability constants can be obtained simply as the quotient of the line slope and the axis intercept of the linear dependence. The relations derived in this paper may be used not only for calculating the stability constants from chromatographic measurements but also for optimizing the process of separation based on the values of the stability constants available in literature