| 高效液相色谱法测定人血清中5-氟尿嘧啶浓度 |
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| 引用本文： | 王冬梅,谢景文.高效液相色谱法测定人血清中5-氟尿嘧啶浓度[J].色谱,1997,15(2):164-165. |
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| 作者姓名： | 王冬梅 谢景文 |
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| 作者单位： | 兰州军区总医院!兰州，730050，兰州军区总医院!兰州，730050 |
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| 摘 要： | 建立了测定人血清中5-氟尿嘧啶的反相高效液相色谱法。采用ZorbaxODS柱4.6mm×250mm，预柱YWG-C184.6mm×50mm;甲醇:水（20:80）为流动相，紫外检测波长为273um，喃氟啶为内标定量。回收率为96.85％，田间和日内变异系数分别为537％和438％，血清中最低检测浓度为10μg／L。在0.04～50mg／L浓度范围内线性良好，相关系数为0.9990。
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| 关 键 词： | 5-氟尿嘧啶 高效波相色谱法 人血清 |
Determination of 5-Fluorouracil in Human Serum by High Performance Liquid Chromatography |
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| Wang Dongmei and Xie Jingwen.Determination of 5-Fluorouracil in Human Serum by High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,1997,15(2):164-165. |
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| Authors: | Wang Dongmei and Xie Jingwen |
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| Institution: | General Hospital of Lanzhou Command of PLA., Lanzhou, 730050. |
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| Abstract: | A reversed-phase HPLC method is described for determination of 5-fluorouracil in human serum. After being extracted with ethyl acetate 5-fluorouracil was analyzed by reversed-phase HPLC colunln (Zorbax ODS)and UV-detector (273nm)with tegaful as internal standard. The mobile phase was a mixture ot meLnyi alcohol and water(20: 80, V:V ) at a flow rate of lmL/min. The linear range was 0. 04-50mg/L(r = 0. 9990) and the detectable limit was 10ng/mL. The average recovery was 96. 85% and the coefficients of variation were 4. 38% and 5. 37 % for within-day and day-to-day tests respectively. |
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| Keywords: | high performance liquid chromatography 5-fluorouracil human serum |
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