首页 | 本学科首页   官方微博 | 高级检索  
     检索      

高效液相色谱法测定朝鲜淫羊藿中淫羊藿甙的含量
引用本文:沙明,曹爱民,杨松松,薛雅民.高效液相色谱法测定朝鲜淫羊藿中淫羊藿甙的含量[J].色谱,1997,15(2):166-167.
作者姓名:沙明  曹爱民  杨松松  薛雅民
作者单位:[1]辽宁中医学院 [2]大连石化公司职工医院
摘    要:采用高效液相色谱法测定朝鲜建羊藿中淫羊藿成的含量,色谱柱为Shim-PackCLC-ODS柱,流动相为乙睛-水(30:70),检测波长270um。在此条件下,淫羊藿式与其它黄酮醇咸的色谱峰分离完全。方法回收率为976%,RSD为1.2%,操作简便,结果可靠,为淫羊藿药材的质量控制提供了新的方法。

关 键 词:朝鲜淫羊藿  高效液相色谱法  淫羊藿甙  

Determination of Icariin in Epimedium Koreanum Nakai by High Performance Liquid Chromatography
Sha Ming,Cao Aimin and Yang Songsong.Determination of Icariin in Epimedium Koreanum Nakai by High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,1997,15(2):166-167.
Authors:Sha Ming  Cao Aimin and Yang Songsong
Institution:Liaoning College of Traditional Chinese Medicine, Shenyang, 110032.
Abstract:An HPLC method for determination of icariin in epimedium koreanum nakai was investigated. The results showed that this method was simple, specific and accurate. The recovery was 97.6% and relative standard deviation was 1.2%. About 0.5 g of dried powdered crude drug was weighed accurately, placed in 25 mL of the mobile phase and refluxed on a water-bath for 30 min. After cooling, the solution was decanted. The residue was refluxed twice with 10 mL of the mobile phase. The extract were placed in a 50 mL volumetric flask and diluted to 50 mL with the mobile phase. A 3 microL of the solution was injected into the HPLC system. Linear calibration graphs for icariin were obtained for the concentration ranges of 0.08-0.4 microg. HPLC conditions were used was Shimadzu LC-6A with UV spectrometric detector SPD-6AV; at 270 nm and auto injector SIL-6A. Water-acetonitrile (70:30) as the mobile phase, Shim-pack CLC-ODS column (6.0 mm x 150 mm).
Keywords:high performance liquid chromatography  epimedium koreanum nakai  icarin  
本文献已被 CNKI 维普 等数据库收录!
点击此处可从《色谱》浏览原始摘要信息
点击此处可从《色谱》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号