高效液相色谱法测定朝鲜淫羊藿中淫羊藿甙的含量

采用高效液相色谱法测定朝鲜建羊藿中淫羊藿成的含量，色谱柱为Shim-PackCLC-ODS柱，流动相为乙睛-水（30:70），检测波长270um。在此条件下，淫羊藿式与其它黄酮醇咸的色谱峰分离完全。方法回收率为976％，RSD为1.2％，操作简便，结果可靠，为淫羊藿药材的质量控制提供了新的方法。

Determination of Icariin in Epimedium Koreanum Nakai by High Performance Liquid Chromatography

An HPLC method for determination of icariin in epimedium koreanum nakai was investigated. The results showed that this method was simple, specific and accurate. The recovery was 97.6% and relative standard deviation was 1.2%. About 0.5 g of dried powdered crude drug was weighed accurately, placed in 25 mL of the mobile phase and refluxed on a water-bath for 30 min. After cooling, the solution was decanted. The residue was refluxed twice with 10 mL of the mobile phase. The extract were placed in a 50 mL volumetric flask and diluted to 50 mL with the mobile phase. A 3 microL of the solution was injected into the HPLC system. Linear calibration graphs for icariin were obtained for the concentration ranges of 0.08-0.4 microg. HPLC conditions were used was Shimadzu LC-6A with UV spectrometric detector SPD-6AV; at 270 nm and auto injector SIL-6A. Water-acetonitrile (70:30) as the mobile phase, Shim-pack CLC-ODS column (6.0 mm x 150 mm).