界面衍生化气相色谱法测定葡萄酒中总有机酸

利用离子交换树脂分离、富集葡萄酒中总有机酸并直接在树脂界面上乙酯化，结合气相色谱／质谱法，鉴定并分析了葡萄酒中２１种有机酸。

Determination of Total Organic Acids in Wine by Interfacial Derivatization Gas Chromatographic Method

This paper presents a new method for analysis of total organic acids in wine as follows: organic acids in wine were separated and enriched with strong anion-exchange resin and were esterified directly on the resin with BF3-diethylether and alcohol for 60 min under the temperature of 90 degrees C. The ethyl esters of organic acids were extracted with dichloromethane and determined by HP 5890A GC-5970B MSD equipped with fused-silica capillary column, 12.5 m x 0. 2mm i.d.. The column temperature was programmed from 30 degrees C to 60 degrees C at 2 degrees C/min, 60 degrees C to 180 degrees C at 5 degrees C/min, 180 degrees C to 230 degrees C at 10 degrees C/min. Twenty one peaks of ethyl esters of organic acids in wine were identified with gas chromatography/mass spectrometry and quantitated with internal standard and working curve. The results of analysis are satisfactory. This paper has provided a useful method of determination of low content of organic acids in complex aqueous samples.