IDA型Cu~(2+):-螯合亲和膜色谱法对牛肝过氧化氢酶纯化的研究(英文):

首次采用以改性纤维素为基质、亚氨二乙酸（ＩＤＡ）为取代配基的铜离子螯合膜色谱法对牛肝过氧化氢酶（ＢＬＣ）的分离纯化进行了研究。缓冲液的ｐＨ值对ＢＬＣ与螯合配基的结合影响显著。在选定的色谱条件下，ＢＬＣ粗酶液经ＩＤＡ型Ｃｕ２＋－螯合膜色谱柱一步纯化，比活性平均提高４．７倍，回收率为６７．７％。金属螯合膜色谱柱可用含０．２ｍｏｌ／Ｌ的咪唑或５０ｍｍｏｌ／ＬＥＤＴＡ－１ｍｏｌ／ＬＮａＣｌ的缓冲液再生，反复使用，后者比前者对柱子的再生效果更好。

Immobilized IDA-type Cu2+-chelating membrane affinity chromatography for purification of bovine liver catalase.

Cu2+-chelating membrane medium based on iminodiacetate-substituted modified short cotton cellulose was examined to purify bovine liver catalase (BLC) by affinity chromatography. The buffer pH notably affect BLC binding to the Cu2+-chelating membrane adsorbent. The results showed that under the chromatographic conditions chosen, 67.7% recovery of BLC attained with an overall 4.7-fold increase in specific activity in a single step. After purification of BLC, the chelating membrane adsorbent can easily be regenerated by imidazole or EDTA buffer. Different results were obtained by the two regeneration methods: the later is more effective than the former in reviving the purification characters of the chelating membrane adsorbent.