高效毛细管电泳法检测尿液中的假尿嘧啶核苷

假尿嘧啶核苷（ψ）主要来自ｔＲＮＡ的降解，已证实在癌症患者尿液中排泄量异常，可以作为肿瘤诊断的极有用的标志物之一。用高效毛细管电泳法快速测定了人尿中的ψ，用涂层柱（２４ｃｍ×２５μｍｉ．ｄ．）及硼酸盐缓冲液，在线２００ｎｍ检测，可在４ｍｉｎ内使ψ与尿苷等及尿中其它内源物质完全分离。方法的日内、日间变异系数均小于４．０％，用磺基水杨酸作内标，以ψ浓度对相应的峰高或峰面积比定量得标准曲线（ｒ＞０．９９９０），ψ最低检测限为４μｍｏｌ／Ｌ。

Determination of Human Urinary Pseudouridine by High Performance Capillary Electrophoresis

Pseudouridine, mainly as a degradation product of transfer ribonucleic acid, has been shown to be excreted in abnormal amounts in the urine of patients with cancer and can be used as one of the potentially valuable tumor markers. In this paper, a method for the rapid determination of human urinary pseudouridine by HPCE is reported. We used a 24 cm x 25 microm i.d. coated capillary and a borate buffer solution (0.1 mol/L, pH 8.6), and set the UV detection wavelength at 200 nm. In this experiment, pseudouridine can be separated completely with uridine and anti-tumor drugs (5-fluouridine and methetrexation) and other endogenous substances in urine within 4 minutes. Assay precision was determined for concentrations of 50 micromol/L and 200 micromol/L as 2.38% and 1.92% for within-day CV, 3.7% and 2.4% for between-day CV respectively. Sulfosalicylic acid was used as internal standard. Linearity, between the concentrations of pseudouridine and the corresponding peak height or area ratios of pseudouridine and the internal standard, was demonstrated in the 6.25-200 micromol/L range (r>0.9990). The detection limit of concentration for pseudouridine was 4 micromol/L. This method, with simple sample preparation, good assay precision, lower cost and full-automation, is an effective means for the assessment of pseudouridine in clinical application.