二极管阵列检测高效液相色谱重叠峰的双波长分析方法

把光度分析的双波长法应用于二极管阵列检测高效液相色谱重叠峰的数据处理。对于色谱峰严重重叠的双组分，只要它们的紫外可见吸收光谱有差异，就可通过计算两个波长下的峰面积（其中一个组分在这两波长时的峰面积之比为常数ｋ，并通过计算另一个组分在ｋ比例系数下的两波长峰面积差）进行定量测定。检测了混合硝基苯类化合物，结果令人满意。

Dual Wavelength Method for Analysis of Overlapped High Performance Liquid Chromatographic Peaks with Multiwavelength Diode Array Detection

High performance liquid chromatography (HPLC) is one of the analytical techniques most frequently used. Its chief limitation lies in the need to avoid the overlap of chromatographic peaks as much as possible. In this work, a new method for the treatment of HPLC data allowing the resolution of extensively overlapped peaks has been developed. This method uses a diode array spectrophotometer and treats the generated data by dual wavelength method. Its foundation is based on calculating the peak areas for two given wavelength in order to determine the contribution of each component to the mixed peak. Because the area is the integration of absorption at different wavelength, the wavelength can be chosen from the absorption spectra of the two compounds for eliminating the interference of the other component in the mixture, and the concentration of the corresponding component can be calculated. This method provides a simplification of the calculation process and a reduction of the amount of the data used. The applicability of the method is checked on mixture of two aromatic nitro compounds. Though the two components almost have the same retention time and their peaks overlapped extensively, the results are satisfactory.