黄酮醇异构体的超临界流体色谱法分离

用超临界流体色谱法进行了黄酮醇异构体的分离研究。考察了温度、压力、流动相组成、柱条件等对分离的影响。在实验的温度范围４０～６０℃和压力范围１５～３０ＭＰａ内，这组化合物都能得到很好的分离；流动相组成是影响色谱分离的最显著的因素，磷酸的加入大大改变了各物质的保留行为；考察了三种硅胶基质键合相对分离的影响，发现苯基柱用于这组异构体的分离最为合适。

Separation of Flavonol Isomers by Packed Column Supercritical Fluids Chromatography

Flavonol isomers, which differ only in the position of hydroxyl group substitute on their chemical structures, showed different chromatographic behaviors. These polar compounds, in general, can be analyzed by high performance liquid chromatography. In this paper, the separation of flavonol isomers by home-set up packed column supercritical fluid chromatograph with carbon dioxide modified by ethanol containing 0.5% (V/V) phosphoric acid as mobile phase is described. The effects of temperature, pressure, composition of mobile phase and packed column type on the separation were studid. The addition of phosphoric acid to the mobile phase, which can not result in corrosion on the chromatographic system, enabled flavonol to elute from the column, and showed that the composition of the mobile phase was the most pronounced factor influencing the separation. Three columns were evaluated for the separation. It showed that the phenyl-bonded silica column was the best and the ODS column was the worst for the isomers separation. Increasing pressure shortened the retention time of each compound with good resolution, and higher temperature led to longer retention time.