Membrane-bound dehydrogenases from Gluconobacter sp.: Interfacial electrochemistry and direct bioelectrocatalysis |
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Authors: | Jan Tkac Juraj Svitel Igor Vostiar Marian Navratil Peter Gemeiner |
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Affiliation: | aDepartment of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, SK 845 38 Bratislava, Slovakia;bInstitute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, SK 812 37 Bratislava, Slovakia |
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Abstract: | Although membrane-bound dehydrogenases isolated from Gluconobacter sp. (mainly PQQ-dependent alcohol and fructose dehydrogenase) have been used for preparing diverse forms of bioelectronic interfaces for almost 2 decades, it is not an easy task to interpret an electrochemical behaviour correctly. Recent discoveries regarding redox properties of membrane-bound dehydrogenases along with extensive investigations of direct electron transfer (DET) or direct bioelectrocatalysis with these enzymes are summarized in this review. The main aim of this review is to draw general conclusions about possible electronic coupling paths of these enzymes on various interfaces via direct electron transfer or direct bioelectrocatalysis. A short overview of the metabolism and respiration chain in Gluconobacter relevant to interfacial electrochemistry is given. Biosensor devices based on DET or direct bioelectrocatalysis using membrane-bound dehydrogenases from Gluconobacter sp. are described briefly with the emphasis given on practical applications of preparing enzymatic biofuel cells. Moreover, interfacial electrochemistry of Gluconobacter oxydans related to the construction of microbial biofuel cells is also discussed. |
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Keywords: | Gluconobacter sp. PQQ-dependent dehydrogenases Direct electron transfer Direct bioelectrocatalysis Biosensors Biofuel cells |
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