Effect of some physico-chemical conditions on an immunoassay for viable Escherichia coli |
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| Authors: | Agnese Jurkevica H. Brian Halsall Carl J. Seliskar William R. Heineman |
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| Affiliation: | (1) Department of Chemistry, University of Cincinnati, P.O. Box 210172, Cincinnati, OH 45221-0172, USA; |
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| Abstract: | Viable Escherichia coli can be detected by an immunoassay in which live bacteria captured on antibody-coated paramagnetic beads are induced to synthesize the enzyme β-galactosidase, which catalyzes the hydrolysis of the slightly fluorescent substrate 4-methyl umbelliferyl-β-d-galactoside to the highly fluorescent product 7-hydroxy-4-methylcoumarin for detection. The effects of bacterial strain, presence of dead bacteria, and some environmental stresses on assay performance were evaluated. |
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