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Effect of some physico-chemical conditions on an immunoassay for viable Escherichia coli
Authors:Agnese Jurkevica  H. Brian Halsall  Carl J. Seliskar  William R. Heineman
Affiliation:(1) Department of Chemistry, University of Cincinnati, P.O. Box 210172, Cincinnati, OH 45221-0172, USA;
Abstract:Viable Escherichia coli can be detected by an immunoassay in which live bacteria captured on antibody-coated paramagnetic beads are induced to synthesize the enzyme β-galactosidase, which catalyzes the hydrolysis of the slightly fluorescent substrate 4-methyl umbelliferyl-β-d-galactoside to the highly fluorescent product 7-hydroxy-4-methylcoumarin for detection. The effects of bacterial strain, presence of dead bacteria, and some environmental stresses on assay performance were evaluated.
Keywords:
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