STREAK CAMERA MEASUREMENT OF TRYPTOPHAN AND RHODAMINE MOTIONS WITH PICOSECOND TIME RESOLUTION |
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| Authors: | T. M. Nordlund,&dagger &dagger D. A. Podolski |
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| Affiliation: | †Department of Radiation Biology and Biophysics and Department of Physics and Astronomy. University of Rochester, Rochester, NY 14642.;*Department of Chemistry and Institute of Optics, University of Rochester, Rochester, NY 14627. USA |
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| Abstract: | We have used a signal averaging laser (30 ps pulse)-streak camera system to measure fluorescence anisotropy decays of dyes in solution and protein tryptophan chromophores with picosecond time resolution. Emission polarization as a function of time can be measured directly and simultaneously for both polarization directions and stored in an optical multichannel analyzer. The corrected anisotropy is computed after background subtraction. Rotations of free dyes in solution (Sulforhodamine 101, tryptophan) and the temperature-dependent internal motions of human serum albumin (tryptophan emission) are displayed. |
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