Partitioning behavior of amino acids in aqueous two-phase systems containing polyethylene glycol and phosphate buffer |
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| Institution: | 1. Key Laboratory of Rare Earths Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China;2. College of Chemistry, Northeast Normal University, Changchun 130024, PR China;1. Biomolecular Interaction Centre, University of Canterbury, PB 4800, Christchurch 8140, New Zealand;2. School of Biological Sciences, University of Canterbury, PB 4800, Christchurch 8140, New Zealand;3. Fonterra Research Centre, Fonterra Co-operative Group Limited, PB 11029, Dairy Farm Road, Palmerston North 4442, New Zealand;4. Department of Chemistry, University of Canterbury, PB 4800, Christchurch 8140, New Zealand;5. Institute of Food, Nutrition and Human Health, Massey University, PB 11 222, Palmerston North 4442, New Zealand;6. Riddet Institute, Massey University, PB 11 222, Palmerston North 4442, New Zealand;7. Callaghan Innovation, Lower Hutt 5040, New Zealand;1. University of Belgrade – Faculty of Chemistry, Innovation Center, Studentski trg 16, 11 000 Belgrade, Serbia;2. University of Belgrade – Faculty of Chemistry, Centre of Excellence in Molecular Food Sciences, Department of Biochemistry, Studentski trg 16, 11 000 Belgrade, Serbia;1. Universidade Tiradentes, Av. Murilo Dantas 300, Farolândia, 49032-490 Aracaju-SE, Brazil;2. Departamento de Química, CICECO, Universidade de Aveiro, 3810-193 Aveiro, Portugal;3. ITP, Instituto de Tecnologia e Pesquisa, Av. Murilo Dantas, 300-Prédio do ITP, Farolândia, 49032-490 Aracaju-SE, Brazil |
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| Abstract: | The partitioning behavior of four amino acids, cysteine, phenylalanine, methionine, and lysine in 15 aqueous two-phase systems (ATPSs) with different polyethylene glycol (PEG) molecular weights and phosphate buffers has been studied in the present paper. The phase diagrams of the systems are investigated together with the effect of the PEG molecular weight and pH of the phosphate solutions. The composition of these systems and some parameters such as density and refractive index are determined. The influences of salts in ATPSs, side chain structure of the amino acids, pH of ATPSs, and the PEG molecular weight on the distribution ratios of the amino acids have been studied. This work is useful for the purification of amino acids and the separation of some proteins whose main surface exposed amino acid residues are these four amino acids, respectively. |
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