离子对色谱法研究吡咯并喹呤醌与D-丝氨酸反应的化学动力学

D-丝氨酸(DSer)作为一种新的神经递质存在于胶质细胞,在中枢神经系统疾病中发挥重要的作用;吡咯并喹呤醌(PQQ)能够促进神经因子的产生和神经保护作用。采用离子对色谱法测定吡咯并喹呤醌(PQQ)与DSer反应中游离PQQ的浓度,通过化学反应动力学研究PQQ与DSer的作用,为PQQ在神经递质的调节作用机制提供重要的信息。采用四丁基溴化铵为离子对试剂,PQQ与DSer孵育反应后各物质在Amethyst C18-P柱上的分离度较好,平均回收率为94.2%～99.3%,相对标准偏差为1.05%～2.03%。PQQ-DSer反应的平均速率常数分别为0.032 h~1 (25 ℃)、0.07 h~1(37 ℃)、0.17 h~1(50 ℃);平均反应活化能为54.7 kJ/mol;半衰期分别为22.0 h(25 ℃)、9.8 h(37 ℃)和3.99 h(50 ℃)。动力学结果显示PQQ对脑内DSer的平衡具有重要的调节作用。该方法简便、可靠。

Study of chemical kinetics between pyrroloquinoline quinine and D-serine in body by ion-pair liquid chromatography

As a new neurotransmitter present in the glial cells, D-serine (DSer) plays an important role in central nervous system diseases. Pyrroloquinoline quinine (PQQ) can promote the production of the nerve growth factor and has a protective effect on nerve injuries. The chemical kinetics of PQQ and DSer was studied by determining the free contents of PQQ using ion-pair liquid chromatography (LC), so it can provide important information for the mechanisms of PQQ in the regulation of neurotransmitter. The PQQ and the production of the incubation were separated on an Amethyst C18-P column using tetrabutylammonium bromide as ion-pair reagent. The average recoveries were between 94.2% and 99.3%, and the relative standard deviations were between 1.05% and 2.03%. The average rate constants (K) of PQQ with DSer were 0.032, 0.07 and 0.17 h(-1) at 25, 37 and 50 degrees C, respectively. The average activation energy (E(a)) was 54.7 kJ/mol. The values of half life (t1/2) were 22.0, 9.8 and 3.99 h at 25, 37 and 50 degrees C, respectively. The results showed that PQQ can regulate the balance of DSer in the brain. The method is simple and reliable.