| 镧系螯合物荧光免疫分析法测定对硫磷 |
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| 引用本文： | 常文保,王海丽.镧系螯合物荧光免疫分析法测定对硫磷[J].分析科学学报,1994,10(2):7-10. |
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| 作者姓名： | 常文保 王海丽 |
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| 作者单位： | 北京大学化学系，北京电力专科学校 |
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| 摘 要： | 本文应用一种新的镧系螯合物荧光标记法研究在药对硫磷的免疫分析测定，并作了血清、尿和水样品的回收分析，检测下限为１００ｐｇ／ｍＬ，回收率８７—１０２％。
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| 关 键 词： | 镧系螯合物 荧光免疫分析 对硫磷 |
Fluoroimmunoassay of Parathion using BSA Carrier Antigen labeled with Europium Chelate of Chlorosulfoylthenoyltrifluoroacetone(CITA) |
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| Wen Bao Chanf,Xiao Da Yang,Yun Xiang CI.Fluoroimmunoassay of Parathion using BSA Carrier Antigen labeled with Europium Chelate of Chlorosulfoylthenoyltrifluoroacetone(CITA)[J].Journal of Analytical Science,1994,10(2):7-10. |
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| Authors: | Wen Bao Chanf Xiao Da Yang Yun Xiang CI |
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| Abstract: | Parathion was conjugated to bovine serum albumin (BSA) and then Eu3+ was stabely incorporated to this BSA-parathion antigen through a new bifunctional chelating ligand-chlorosulfoylthenoyltrifluoroactone(CTTA)By using this labeled antigen.a competitive lmmunoassay of parathion was developed:The wells of microtitration plate were coated with rabbit anti-parathion serum;After immunoabstraction of sample parathion.a poper amount of labded antigen was added to each well for competition;And finally Eu3+ was dissociated from immunocomponents on the solids surface by an enhancement solution and the fluorescence were measured.The detection limit of parathion was about 0.1ug/mL and recoveries in serum, urine and water were 87% 102% and 101%.respectively. |
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| Keywords: | Fluoroimmunoassay CTTA parathion Europium chelate |
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