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反相离子对高效液相色谱法测定大白鼠牙髓中5-羟色胺
引用本文:崔冶建,付亚平,李阳,彭彬.反相离子对高效液相色谱法测定大白鼠牙髓中5-羟色胺[J].色谱,1995,13(3):231-234.
作者姓名:崔冶建  付亚平  李阳  彭彬
作者单位:湖北医科大学实验中心,湖北口腔医学院
摘    要:A high performance liquid chromatographic method with electrochemical detection for measuring 5-hy-droxytryptamine(5-HT) in the dental pulp of white rat is presented in the paper.Protein was precipitated by3.3%trichloroacetic acid and 200μL of supernatant was injected.Chromatography was performed on aYWG-C(18) column. Isoproterenol was used as internal standard.The calibration curve was linear within therange of 0. 125~2ng/mL.

关 键 词:5-羟色胺  大白鼠牙髓  高效液相色谱  离子对色谱  

Assay of 5-Hydroxytryptamine in the Dental Pulp of White Rat UsingIon-pair Reversed-Phase High Performance Liquid Chromatography
Cui Yejian,Fu Yaping,Li Yang and Peng Bin.Assay of 5-Hydroxytryptamine in the Dental Pulp of White Rat UsingIon-pair Reversed-Phase High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,1995,13(3):231-234.
Authors:Cui Yejian  Fu Yaping  Li Yang and Peng Bin
Abstract:
Keywords:ion-pair chromatography  high performance liquid chromatography  5-hydroxytryptamine  dental pulp of white rat  
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