Color transitions in coral's fluorescent proteins by site-directed mutagenesis |
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| Authors: | Nadya G Gurskaya Alexander P Savitsky Yurii G Yanushevich Sergey A Lukyanov Konstantin A Lukyanov |
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| Affiliation: | (1) Shemiakin and Ovchimiikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, 117871 Moscow, Russia;(2) Institute of Biochemistry RAS, Leninsky pr. 33, 117071 Moscow, Russia |
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| Abstract: | Background Green Fluorescent Protein (GFP) cloned from jellyfish Aequorea victoria and its homologs from corals Anthozoa have a great practical significance as in vivo markers of gene expression. Also, they are an interesting puzzle of protein science due to an unusual mechanism of chromophore formation and diversity of fluorescent colors. Fluorescent proteins can be subdivided into cyan (~ 485 nm), green (~ 505 nm), yellow (~ 540 nm), and red (>580 nm) emitters. |
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