Room-temperature luminescence optosensings based on immobilized active principles actives: Application to nafronyl and naproxen determination in pharmaceutical preparations and biological fluids |
| |
Authors: | J.F Ferná ndez-Sá nchezA Segura-Carretero,C Cruces-BlancoA Ferná ndez-Gutié rrez |
| |
Affiliation: | Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/Fuentenueva s/n, E-18071 Granada, Spain |
| |
Abstract: | This paper presents two easy and selective methods for determining the active principles nafronyl (NFL) and naproxen (NAP), using a flow-through fluorescence optosensor based on the on-line immobilization on a nonionic-exchanger (Silica Gel, Davisil™ and Amberlite XAD 7, respectively) solid support. The determination was performed in 5×10−3 M HAc/NaAc buffer solution at pH 5 for NFL and 15×10−3 M glycine/HCl buffer solution at pH 2.5 for NAP at a working temperature of 20 °C. The fluorescence intensities were measured at λex/em=294/336 nm and λex/em=332/354 nm for NFL and NAP, respectively. The response time for these optosensors were practically instant, obtaining a linear concentration range between 0 and 700.0 ng ml−1 with a detection limit of 20.8 ng ml−1, an analytical sensitivity of 10.1 ng ml−1 and a standard deviation of 1.27% at a 500 ng ml−1 concentration level for NFL and a linear concentration range between 0 and 200.0 ng ml−1 with the detection limit of 13.3 ng ml−1, an analytical sensitivity of 6.0 ng ml−1 and a standard deviation of 3.52% at a 100 ng ml−1 concentration level for NAP. The proposed methods were satisfactorily applied to real samples (three commercial formulations and urine samples). The effects of the possible interferences were evaluated in all cases. |
| |
Keywords: | Fluorescence optosensor Nafronyl Naproxen Pharmaceutical preparations Urine |
本文献已被 ScienceDirect 等数据库收录! |
|