Immobilization of oligonucleotides onto silica nanoparticles for DNA hybridization studies |
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Authors: | Lisa R HilliardXiaojun Zhao Weihong Tan |
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Affiliation: | Center for Research at Bio/Nano Interface, Department of Chemistry, and the McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA |
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Abstract: | This paper describes the development of oligonucleotide-functionalized nanoparticles. We used disulfide-coupling chemistry for the immobilization of oligonucleotides onto silica nanoparticles and subsequently demonstrated the properties of the resulting DNA nanoparticles. Factors influencing the immobilization and hybridization processes were examined and optimized. The oligonucleotide-modified silica nanoparticles provide an efficient substrate for hybridization and can be used in the development of DNA biosensors and biochips. |
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Keywords: | Covalent immobilization Oligonucleotides Silica nanoparticles Disulfide-coupling Thiol/disulfide exchange reaction |
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