Determination of sophoridine and related lupin alkaloids using tris(2,2′-bipyridine)ruthenium electrogenerated chemiluminescence |
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| Authors: | Xi Chen Changqing YiMeijin Li Xin LuZhen Li Peiwei LiXiaoru Wang |
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| Institution: | a The Key Laboratory of Analytical Sciences of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, PR China
b State Key Laboratory for Physical Chemistry of Solid Surfaces, Department of Chemistry, Xiamen University, Xiamen 361005, PR China |
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| Abstract: | Electrogenerated chemiluminescences (ECLs) based on tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy)32+) and lupin alkaloids, for instance, sophoridine (SRI), matrine (MT), sophoranol (SR) and sophocarpine (SC) in an aqueous alkaline buffer solution (pH 9.0) are studied. The light emission is mainly caused by an electro-oxidation reaction between tertiary amino group on the alkaloid compounds and Ru(bpy)32+ in a thin layer flow cell equipped with a glassy carbon disc electrode (22.1 mm2) at the potential of +1.50 V (versus Ag/AgCl). The luminescence wavelength of 610 nm confirmed that ECL is caused by Ru(bpy)32+∗ to its ground state. ECL intensities of these lupin alkaloids are affected by the substituent character, three-dimensional conformation of hydrogen on β-carbon atom. Ionization potentials taken from calculation data further confirm the experimental results. In addition, the factors affecting the determination and HPLC separation of the four alkaloids are also investigated. |
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| Keywords: | Electrogenerated chemiluminescence Lupin alkaloids Tris(2 2&prime -bipyridyl)ruthenium HPLC analysis |
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