Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides |
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Authors: | Silvana Andreescu Lise BarthelmebsJean-Louis Marty |
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Affiliation: | a Centre de Phytopharmacie, Universite de Perpignan -URA 5054, 52 Avenue de Villeneuve, 66860 Perpignan Cedex, France b Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, 90-92 Sos. Panduri, 76234 Bucharest, Romania |
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Abstract: | The analytical performance of three acetylcholinesterase (AChE) screen-printed biosensors designed for the detection of pesticides are evaluated. Bioencapsulation of the enzyme in a sol-gel composite and immobilization by metal-chelate affinity were compared with the entrapment of the enzyme in a photopolymerisable polymer. A very low amount of enzyme ranging between 0.8 and 1.2 mIU was immobilized on the electrode surface in each approach. The sensors exhibited a storage stability of over 6 months when the enzyme was encapsulated in a polymer film. Pesticide concentrations in the range of 10−8 to 10−9 M paraoxon, dichlorvos and chlorpyrifos ethyl oxon could be detected according to each configuration by following an incubation time of 20 min. |
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Keywords: | Biosensor Acetylcholinesterase Immobilization Screen-printed Pesticides |
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