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Performance of amperometric alcohol electrodes prepared by plasma polymerization technique
Authors:Dilek ÇökelilerMehmet Mutlu
Institution:Department of Food Engineering, Engineering Faculty, Hacettepe University, Beytepe Campus, TR 06532 Ankara, Turkey
Abstract:A single-layer alcohol electrode was prepared by plasma polymerization technique. Ethylenediamine was used to incorporate amino groups on tract-etched polycarbonate membranes in glow discharge reactor. In order to determine the plasma polymerization parameters (discharged power, monomer flow rate, exposure time) on membrane permeability, hydrogen peroxide was used as tracer. The single-layer alcohol electrode that was produced by 0.6% (w/v) of alcohol oxidase (AOx) solution on the polycarbonate membrane, which was modified at 30 W, 20 ml/min monomer flow rate and 15 min exposure time, was selected for optimum performance. Sensitivity, linearity and response time of that particular layer were 5.6 nA/mM, 2 mM and 50 s, respectively. The performance of the amperometric alcohol electrode was tested on commercial alcoholic beverages.
Keywords:Plasma polymerization  Enzyme electrode  Alcohol electrode  Biosensor
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