| 离子液体对植物水解酶抑制法测定克百威的影响 |
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| 引用本文： | 何丽君,伍艳,谢洪学,卢奎.离子液体对植物水解酶抑制法测定克百威的影响[J].分析科学学报,2008,24(3):341-343. |
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| 作者姓名： | 何丽君 伍艳 谢洪学 卢奎 |
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| 作者单位： | 河南工业大学化学化工学院,郑州,450001 |
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| 基金项目： | 河南省科技攻关项目
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河南省自然科学基金
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河南省高校青年骨干教师资助项目 |
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| 摘 要： | 考察了N-丁基吡啶四氟硼酸盐(BPy]BF4])和1-丁基-3-甲基咪唑四氟硼酸盐(BMim]BF4])离子液体对植物水解酶抑制法测定克百威的影响(酶活性、抑制率、分析时间和检出限)。与未添加离子液体的磷酸缓冲溶液体系相比,农药对酶的抑制率在8%(V/V)BPy]BF4]-磷酸缓冲溶液体系中提高了2.2倍,克百威的检出限降低了约1个数量级;同时加快了农药对酶的抑制速度,使分析时间缩短了4 min。添加BMim]BF4]离子液体有类似的影响。
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| 关 键 词： | 离子液体 克百威 植物水解酶 |
| 文章编号： | 1006-6144(2008)03-0341-03 |
| 修稿时间： | 2007年4月3日 |
Determination of Carbofuran Using Ionic Liquid-based Plant Lipases Inactivation Method |
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| HE Li-jun,WU Yan,XIE Hong-xue,LU Kui.Determination of Carbofuran Using Ionic Liquid-based Plant Lipases Inactivation Method[J].Journal of Analytical Science,2008,24(3):341-343. |
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| Authors: | HE Li-jun WU Yan XIE Hong-xue LU Kui |
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| Abstract: | The influences of N-butylpyridinium tetrafluoroborate() and 1-butyl-3-methyl-imidazolium tetrafluoroborate() ionic liquids on determining carbofuran by plant lipases inactivation method were investigated.Some factors,such as enzyme activity,inhibition officiency,analysis time and detection limit,were evaluated.The enzyme inhibition efficiency in 8% -phosphate buffer mixtures was increassed by 2.2 times companing with that in phosphate buffer,while the analysis time was shortened by 4 min,and the detection limit of caxbofuran was lowered by one order of magnitude.The similar results were obtained for . |
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| Keywords: | Ionic liquid Carbofuran Plant lipases inactivation |
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