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Development of a fast spectroscopic enzyme assay for on-site measurement of total polyphenol content in grapes and wine
Authors:Daniela Harkensee  Sascha Beutel  Manuel Young  Roland Ulber
Affiliation:1. Institut für Technische Chemie, Universit?t Hannover, Callinstr. 3, 30167, Hannover, Germany
2. Centro de Biotecnología, Universidad Téchnica Federico Santa Maria, Av. Espana 1680, Valparaiso, Chile
3. FB Maschinenbau und Verfahrenstechnik, Lehrgebiet Bioverfahrenstechnik, Technische Universit?t Kaiserslautern, Gottlieb-Daimler-Straβe, 67663, Kaiserslautern, Germany
Abstract:In recent years interest in polyphenols as a nutrient in vegetables and fruits has increased because of polyphenols’ positive effects on human health. The interest focuses on the sensory properties of polyphenols and their influence on the taste of fruits and derived products. This article presents the development of a bioanalytical measurement technique enabling the determination of the total polyphenol content (TPC) of fresh grapes within a few minutes. Furthermore this technique allows the control of TPC during production processes, e. g. fermentation of wine.
Keywords:Polyphenols  Enzyme assay  Wine  Grapes  Analysis
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