| 高效液相色谱-荧光分析法测定人体尿液中的蝶呤-6-羧酸 |
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| 引用本文： | 万益群,陈林,谭婷.高效液相色谱-荧光分析法测定人体尿液中的蝶呤-6-羧酸[J].南昌大学学报(理科版),2009,33(5):1. |
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| 作者姓名： | 万益群 陈林 谭婷 |
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| 作者单位： | 南昌大学分析测试中心； |
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| 基金项目： | 国家自然科学基金资助项目，江西省自然科学基金资助项目 |
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| 摘 要： | 建立了高效液相色谱-荧光法测定人体尿液中蝶呤-6-羧酸的分析方法。以乙腈-水(5:95,v/v)为流动相,流速为0.8 mL/min,荧光检测波长为:λex=360 nm,λem=440 nm。尿样经0.45μm的微孔滤膜过滤后,按选定的色谱条件进行分析,蝶呤-6-羧酸含量在(0.007~2.4)μg/mL范围内呈良好的线性
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| 关 键 词： | 高效液相色谱 荧光检测 蝶呤-6-羧酸 尿液 |
Determination of Pterin-6-carboxylic Acid in Human Urine by HPLC with Fluorescence Detection |
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| WAN Yi-qun,CHEN Lin,TAN Ting.Determination of Pterin-6-carboxylic Acid in Human Urine by HPLC with Fluorescence Detection[J].Journal of Nanchang University(Natural Science),2009,33(5):1. |
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| Authors: | WAN Yi-qun CHEN Lin TAN Ting |
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| Abstract: | The high-performance liquid chromatography (HPLC) with fluorescence detection method was developed for determination of pterin-6-carboxylic acid in human urine.The conditions of HPLC were the following:the mobile phase was acetonitril-water (5:95,v/v),the flow rate was 0.8 mL/min,the excitation and emission wavelengths were 360 nm and 440 nm,respectively.The linear range was (0.007～2.4) μg/mL.The linear regression equation was y=394.55x+1.24(r=0.999 9),with the detection limit of 0.002μg/mL.For pterin-6- carboxylic acid in human urine,the recoveries were in the range of 101.3%-104.6 % and the relative standard derivation (RSD) was less than 7.1%.The proposed method was successfully applied to the determination of pterin-6-carboxylicacid in human urine. |
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| Keywords: | pterin-6- carboxylicacid urine HPLC fluorescence detection |
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