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| Silver nanoparticle labeled immunoresonance scattering spectral assay for trace fibrinogen | |
| 作者单位： | JIANG ZhiLiang（School of Environment and Resource, Guangxi Normal University, Guilin 541004, China;Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China） ;CHEN YuanYuan（School of Environment and Resource, Guangxi Normal University, Guilin 541004, China） ;LIANG AiHui（Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China） ;TAO HuiLin（Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China） ;TANG NingLi（Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China） ;ZHONG FuXin（Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China） ; |
| 基金项目： | 国家自然科学基金;广西新世纪十百千人才工程基金 |
| 摘 要： | Silver nanoparticles in size of 8.0 nm was prepared by the trisodium citrate and used to label goat anti-human fibrinogen. In the pH 5.8 Na2HPO4-NaH2O4 buffer solution (PBS) and in the presence of polyethylene glycol (PEG) and KCl, the immune reaction between silver-labeled goat anti-human fibrinogen and fibrinogen took place and led the resonance scattering intensity at 465 nm (I465) to decreasing. The I465 decreased intensity was linear to the fibrinogen concentration in the range from 0.067 to 1.67 μg/mL, with a detection limit of 0.024 μg/mL. This method was applied to determination of fibrinogen in human plasma, with satisfactory results. |
| 收稿时间： | 2005-09-23 |
| 修稿时间： | 2006-09-06 |
Silver nanoparticle labeled immunoresonance scattering spectral assay for trace fibrinogen |
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| Jiang ZhiLiang,Chen YuanYuan,Liang AiHui,Tao HuiLin,Tang NingLi,Zhong FuXin. Silver nanoparticle labeled immunoresonance scattering spectral assay for trace fibrinogen[J]. Science in China(Chemistry), 2007, 50(3): 345-350. DOI: 10.1007/s11426-007-0064-2 | |
| Authors: | Jiang ZhiLiang Chen YuanYuan Liang AiHui Tao HuiLin Tang NingLi Zhong FuXin |
| Affiliation: | 1. School of Environment and Resource, Guangxi Normal University, Guilin 541004, China;Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China 2. School of Environment and Resource, Guangxi Normal University, Guilin 541004, China 3. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China |
| Abstract: | Silver nanoparticles in size of 8.0 nm was prepared by the trisodium citrate and used to label goat anti-human fibrinogen. In the pH 5.8 Na2HPO4-NaH2O4 buffer solution (PBS) and in the presence of polyethylene glycol (PEG) and KCl, the immune reaction between silver-labeled goat anti-human fibrinogen and fibrinogen took place and led the resonance scattering intensity at 465 nm (I 465) to decreasing. The I 465 decreased intensity was linear to the fibrinogen concentration in the range from 0.067 to 1.67 μg/mL, with a detection limit of 0.024 μg/mL. This method was applied to determination of fibrinogen in human plasma, with satisfactory results. Supported by the National Natural Science Foundation of China (Grant No. 20365001) and the Guangxi Foundation of New Century Ten-Hundred-Thousand Talents |
| Keywords: | silver-nanoparticle fibrinogen goat anti-human immune fibrinogen serum resonance scattering spectral assay |
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