Coupling chemiluminescence with capillary electrophoresis to analyze single human red blood cells |
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Authors: | Zhi Qing Xie Chao Huang Xiangyi Ren Jicun |
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Institution: | College of Chemistry and Chemical Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, PR China |
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Abstract: | In this paper, we describe a new method for determination of hemoglobin of single red blood cells by coupling chemiluminescence with capillary electrophoresis (CL-CE). The chemiluminescent detection is based on the catalytic effects of hemoglobin on the luminol-hydrogen peroxide reaction. The conditions of chemiluminescent reaction and capillary electrophoresis were investigated. Hemoglobin in human blood samples was detected with the present method, the linear range from 1.7 μg mL−1 to 6.8 μg mL−1 was tested, and the correlation coefficient of 0.997 and low detection limit of 0.17 μg mL−1 (approximately 2.2 pg, S/N = 3) were obtained. Cell injection procedure was improved, and the method was successfully used to determine hemoglobin of single red blood cells and the statistical result of the average content of hemoglobin in 26 human red blood cells was 23.6 pg. Compared to other current methods, CE with CL system is simple, sensitive and will become an attractive alternative method for single cell analysis. |
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Keywords: | Single cell analysis Red blood cell Hemoglobin Chemiluminescence Capillary electrophoresis |
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