Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor |
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| Authors: | Singh Suman Singhal Rahul Malhotra B D |
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| Affiliation: | a Central Mechanical Engineering Research Institute, G. Avenue, Durgapur 713209, West Bengal, India
b Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India |
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| Abstract: | Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) have been covalently immobilized onto tetraethylorthosilicate (TEOS) sol-gel films. The tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films thus prepared have been characterized using scanning electron microscopic (SEM), UV-vis spectroscopic, Fourier-transform-infrared (FTIR) spectroscopic and amperometric techniques, respectively. The results of photometric measurements carried out on tetraethylorthosilicate sol-gel/ChEt/ChOx reveal thermal stability up to 55 °C, response time as 180 s, linearity up to 780 mg dL−1 (12 mM), shelf life of 1 month, detection limit of 12 mg dL−1 and sensitivity as 5.4 × 10−5 Abs. mg−1 dL−1. |
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| Keywords: | Tetraethylorthosilicate (TEOS) sol-gel Cholesterol oxidase Cholesterol esterase Cholesterol oleate Encapsulation Cholesterol biosensor |
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